The book Dastur al-Shu'ara is one of the comprehensive and instructive works in the field of literary sciences and the art of poetry. This book was authored in 1048 AH by Amani Mazandarani and dedicated to Mirza Muhammad Taqi, a minister of the time. The core discussions of the book revolve around the definition of poetry and the poet, as well as chapters on various topics such as prosody (aruz), rhyme (qafiya), rhetorical devices (badi'), and issues related to poetic plagiarism (saraqat-e shi'ri). The comprehensive and detailed information provided on each of these topics adds to the significance of the book. It can be confidently stated that this book was capable of addressing the needs of poets in understanding the formal and structural aspects of poetry, and it remains highly relevant even today. The book includes numerous poetic examples, containing a wealth of verses from poets contemporary to the author, which can serve as a reflection of the poetic standards of that era. The linguistic and stylistic features of Dastur al-Shu'ara largely follow the scientific prose style of the Safavid period. In this article, out of the twenty-four existing manuscripts of the book, twenty-three are briefly introduced, and manuscript No. 4342 from the Astan Quds Library, which was transcribed during the author's lifetime, is examined in detail. This article not only introduces the manuscript of the book but also provides a structural and content analysis, offering insights into its various aspects and critiques.